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Watch how Test4D works



Check Vitamin D Deficiency Before Other Causes of a Disease

Vitamin D deficiency is a global pandemic. In the Philippines, 7 out of 10 were found out to be Vitamin D deficient. Their Vitamin D in blood is less than optimal to prevent chronic diseases.

Vitamin D is actually a hormone. That is why low Vitamin D is risk factor not only for bone illness but also for cardiovascular disease, cognitive impairment, severe asthma, diabetes and cancer. More research has been done on Vitamin D than on Vitamins A, C, E and K combined.

Know your Vitamin D levels. Most don’t know they’re Vitamin D deficient. The symptoms are subtle and non-specific. When it comes to your health, leave no room for guesswork. Now there’s a simple and quick way to check Vitamin D status. Test4D.

Point of Care Testing

Point-of-Care-Testing (POCT) is defined as medical testing at or near the site of patient care. The driving notion behind POCT is to bring the test conveniently and immediately to the patient. This increases the likelihood that the patient, physician and care team will receive the results quicker, which allows for immediate clinical decisions to be made.

About Test4D™

Test4D™ is a rapid chromatographic immunoassay for the detection of Vitamin D levels in blood at a cut off concentration of 32 ± 6 ng/mL (80 ±15 nmol/L). It is the first and only true Point-of-Care-Testing that allows you to safely test Vitamin D sufficiency or deficiency on the spot, without any equipment.

Test4D™ Box Composition

Each box is good for 25 tests and contains the following:

  • Osteoporosis, Osteoarthritis and other bone related diseases
  • 25 individually packed test devices in foil pouches
  • 25 disposable transfer pipettes – droppers
  • 25 lancets
  • 1 buffer vial (5 mL)

Principle Behind Test4D™

  • The test is based on the competition between the natural Vitamin D present in blood sample and the labeled Vitamin D present in the test strip for the Vitamin D binding antibody (competitive enzyme immunoassay).
  • Before testing, there are two invisible parallel lines in the test kit, a Control Line and a Test Line. The Test Line contains immobilized monoclonal anti-body gold conjugate that can bind specifically to 25-OH Vitamin D, either natural or labeled.

  • During testing, the natural Vitamin D in the blood sample competes with the labeled Vitamin D in the test strip for the limited number of antibody-gold conjugate. An amount in the equivalent of 32 ng/mL or more of natural Vitamin D in the blood sample completely binds all antibodies, and prevents the Test Line from turning red (Sufficient Vitamin D Level).

  • If natural Vitamin D in the blood sample is less than the equivalent of 32 ng/mL, there will be free antibodies in the Test Line that can bind to the labeled Vitamin D in the test strip. Binding of labeled Vitamin D with the antibodies will turn the Test Line red (Deficient Vitamin D Level).

  • The Control Line is the procedural control. This colored line should always appear if the test device is stored in good condition and if the test is performed appropriately